Stoichiometry of the interaction of lima bean protease inhibitor with trypsin and/or chymotrypsin
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چکیده
منابع مشابه
Fluorescence polarization decay of tyrosine in lima bean trypsin inhibitor.
The fluorescence of lima bean trypsin inhibitor is due to a single tyrosine residue at position 69. The lifetime of this tyrosine fluorescence is 620 +/- 50 ps (mean +/- SD) and is little affected by addition of 0.88 M citrate, an efficient quencher of tyrosine fluorescence. The steady-state emission intensity is also only weakly reduced by the quencher. The tyrosine is thus not accessible to t...
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A 516-bp winged bean chymotrypsin-trypsin inhibitor (WbCTI) gene was amplified from genomic DNA and cDNA isolated from winged bean using a pair of degenerate primers designed on the basis of the amino acid sequences of WbCTI. The amplified PCR products were cloned and sequenced to confirm their authenticity. DNA sequence analysis of the genomic and cDNA clones of WbCTI revealed the same nucleot...
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The interaction ae-macroglobulin with four proteinases has been investigated by binding assays and by gel electrophoresis. At pH 7.65 the binding ratios of the proteinase-cwa-macroglobulin complexes were found to be 2:l (trypsin and papain), 1.4:1 (chymotrypsin), and 1:l (plasmin). The progressive decrease in the stoichiometry of the three seryl proteinase complexes was paralleled by a concomit...
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In present studies several attempts have been made to reduce the amount of trypsin and chymotrypsin inhibitors in winged bean (Psophocarpus tetragonolobus) mutants developed through induced mutation. The low seed TI (trypsin inhibitor) lines comprised long pod-5 and long pod-2 mutant lines. The low tuber TI feature could be noted in the mutant population. Such lines were long pod-5, large leaf/...
متن کاملReactions of Trypsin and Chymotrypsin with Heparin, Trypsin Inhibitor, and Hexylresorcinol
il study of enzyme inhibitors designed to obtain information on the possible physiological significance of blood antiprotease has produced a number of simple fundamental reactions which have hitherto not been reported. It is the purpose of this paper to record these reactions for the possible benefit of others who may wish to investigate them further. Materials-The t,rypsin, chymotrypsin, and t...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1971
ISSN: 0014-5793
DOI: 10.1016/0014-5793(71)80255-7